Seed longevity is essential for agricultural productivity and the conservation of genetic resources. Temperature and relative humidity are the primary factors influencing seed viability during storage. Proper management of these conditions reduces post-harvest losses, maintains germination, and extends seed shelf life. This article discusses the scientific basis of seed longevity, physiological responses to storage conditions, and practical strategies for optimizing seed storage. It also highlights the importance of species-specific storage requirements and advances in storage technologies for preserving seed quality, supporting sustainable agriculture, and safeguarding germplasm for future breeding programs.